Bo Huang          Curriculum vitae         Full Version

1700 4th St, MC 2532
Byers Hall 303A
San Francisco, CA 94143

Tel: (415) 476-1866
Fax: (415) 514-1028


Investigator, Chan Zuckerberg Biohub 2017-
Professor, Department of Pharmaceutical Chemistry and Department of Biochemistry and Biophysics, UCSF 2017-
Associate Professor, Department of Pharmaceutical Chemistry and Department of Biochemistry and Biophysics, UCSF 2014-2017
Assistant Professor, Department of Pharmaceutical Chemistry and Department of Biochemistry and Biophysics, UCSF 2009-2014

Academic Training

Harvard University
    Postdoctoral Fellow                     Mentor: Xiaowei Zhuang
Stanford University
    Ph.D. in Chemistry                      Advisor: Richard N. Zare
    Thesis: Chemical analysis of single cells
Peking University, China
    B.S. in Chemistry summa cum laude

Honors Received

◊  UCSF Byers Award in Basic Science 2017
◊  Chan Zuckerberg Biohub Investigator 2017-2022
◊  Finalist, Blavatnik National Awards for Young Scientists 2016-2018
◊  ASCB Early Career Life Scientist Award 2016
◊  W.M. Keck Foundation Medical Research Grant 2014
◊  NIH Director's New Innovator Award 2011
◊  Packard Fellowship for Science and Engineering 2010
◊  Searle Scholars 2010
◊  GE & Science Young Life Scientist Prize
    North American Regional Winner
◊  Annual Reviews Prize in Physical Chemistry,
    Stanford University
◊  Stanford Graduate Fellowship 2001-2004
◊  Rado Young Genius Prize in China, First Place 1996

Selected Publications

  • 98. Y. Wang, H. Pinkard, E. Khwaja, S. Zhou, L. Waller, B. Huang, "Image denoising for fluorescence microscopy by supervised to self-supervised transfer learning", Opt. Express 29, 41303-41312 (2021).
  • 95. X. Shi*, Q. Li*, Z. Dai, A.A. Tran, S. Feng, A.D. Ramirez, Z. Lin, X. Wang, T.T. Chow, J. CHen, D. Kumar, A.R. McColloch, J.F. Reiter, E.J. Huang, I.B. Seiple#, B. Huang#, "Label-retention expansion microscopy", J. Cell Biol., 220, e202105067 (2021).
  • 92. A. Tulpule*, J. Guan*, D.S. Neel*, H.R. Allegakoen, Y.P. Lin, D. Brown, Y. Chou, A. Heslin, N. Chatterjee, S. Perati, S. Menon, T.A. Nguyen, J. Debnath, A.D. Ramirez, X. Shi, B. Yang, S. Feng, S. Makhija, B. Huang#, T.G. Bivona#, "Kinase-mediated RAS signaling via membraneless cytoplasmic protein", Cell 184, 2649-2664.e18 (2021).
  • 91. S. Makhija, D. Brown, R.M. Rudlaff, J. Doh, S. Bourke, Y. Wang, S. Zhou, R. Cheloor-Kovilakam, B. Huang, "Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation", ACS Chem. Biol. 16, 671-681 (2021).
  • 82. B. Yang, X. Chen, Y. Wang, S. Feng, V. Pessino, N. Stuurman, N.H. Cho, K.W. Cheng, S.J. Lord, L. Xu, D. Xie, R.D. Mullins, M.D. Leonetti, B. Huang, "Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution", Nat. Methods 16, 501-504 (2019).
  • 78. B. Chen, W. Zou, H. Xu, Y. Liang, B. Huang, "Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag", Nat. Commun. 9, 5065 (2018).
  • 73. J. Schnitzbauer, Y. Wang, M. Bakalar, B. Chen, T. Nuwal, S. Zhao, B. Huang, "Correlation analysis framework for localization-based superresolution microscopy", Proc. Nat. Acad. Sci. USA 115, 3219-3224 (2018).
  • 70. X. Shi, G. Garcia, J.C. Van De Weghe, R. McGorty, G.J. Pazour, D. Doherty, B. Huang*, J.F. Reiter*, "Super-resolution microscopy reveals that disruption of ciliary transition-zone architecture causes Joubert syndrome", Nat. Cell Biol. 19, 1178-1188 (2017).
  • 69. R. McGorty, D. Xie, B. Huang, "High-NA open-top selective-plane illumination microscopy for biological imaging", Opt. Express, 25, 17798-17810 (2017).
  • 68. S. Feng, S. Sekine, V. Pessino, H. Li, M.D. Leonetti, B. Huang, "Improved split fluorescent proteins for endogenous protein labeling", Nat. Commun., 8, 370 (2017).
  • 63. J. Guan, H. Liu, X. Shi, S. Feng, B. Huang, "Tracking multiple genomic elements using correlative CRISPR imaging and sequential DNA FISH", Biophys. J., 112, 1077-1084 (2017).
  • 56. M.D. Leonetti, S. Sekine, D. Kamiyama, J.S. Weissman, B. Huang, "A scalable strategy for high-throughput GFP tagging of endogenous human proteins", Proc. Nat. Acad. Sci. USA, 113, E3501-E3508 (2016).
  • 53. D. Kamiyama, S. Sekine, B. Barsi-Rhyne, J. Hu, B. Chen, L.A. Gilbert, H. Ishikawa, M.D. Leonetti, W.F. Marshall, J.S. Weissman, B. Huang, "Versatile protein tagging in cells with split fluorescent protein", Nat. Commun., 7, 11046 (2016).
  • 51. B. Chen, J. Hu, R. Almeida, H. Liu, S. Balakrishnan, C. Covill-Cooke, W.A. Lim, B. Huang, "Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci", Nucleic Acids Res. 44, e75 (2016).
  • 50. D. Kamiyama, R. McGorty, R. Kamiyama, M.D. Kim, A. Chiba, B. Huang, "Specification of dendritogenesis site in Drosophila aCC motoneuron by membrane-enrichment of Pak1 through Dscam1", Dev. Cell, 35, 93-106 (2015).
  • 42. B. Chen, L.A. Gilbert, B.A. Cimini, J. Schnitzbauer, W. Zhang, G.W. Li, J. Park. E.H. Blackburn, J.S. Weissman, L.S. Qi, B. Huang, “Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system”, Cell, 155, 1479-1491 (2013).
  • 40. E.M. Puchner, J.M. Walter, R. Kasper, B. Huang*, W.A. Lim*, “Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory”, Proc. Nat. Acad. Soc. USA, 110, 16015-16020 (2013).
  • 31. L. Zhu, W. Zhang, D. Elnatan, B. Huang, “Faster STORM with compressed sensing”, Nat. Methods, 9, 721-723 (2012).

Professional Activities

  • Instructor, 2010 Bangalore Microscopy Course
  • Instructor, 2011, 2013, 2016, 2017 Cold Spring Harbor Laboratory Course in Quantitative Imaging
  • Co-organizer, 2012-2014 QB3-UCSF Course on Biological Microscopy
  • Member, Biophysical Society (2002-Date)
  • Member, American Chemistry Society (2005-Date)
  • Member, American Society of Cell Biology (2008-Date)
  • Journal Reviewer: Cell, Nature, Science, Nature Methods, Nature Biotechnology, Nature Cell Biology, Nature Communications, PNAS, eLife, Developmental Cell, Journal of American Chemical Society, Structure, Nano Letter, Nanoscale, Journal of Molecular Biology, Biophysical Journal, Optica, Optics Letter, Optics Express, Biomedical Optics Express, Microscopy Research and Technique, PLoS One, Scientific Report, etc.